This training class builds on the earlier information for introductory PFAS topics presented in the PFAS 101 CLU-IN training. It provides more in-depth information for human health effects, ecological toxicity and ecological risk assessment, PFAS regulations, and AFFF alternatives and replacement. Resources and further details for the topics included in this training are in the published PFAS-1 guidance document in Sections 7, 8 and 17.2 along with referenced tables. Learning Objectives: Discuss new information on the health effects of categories of PFAS not previously addressed. We'll also talk about the recent use of data from epidemiology studies to develop toxicity factors and guidelines; describe how the U.S. EPA has recently used a mixtures approach to derive a proposed MCL for 4 PFAS. We'll discuss why certain agencies are regulating PFAS as a class, and give examples, and will also give brief updates on our understanding of the adverse effects of PFAS after inhalation or dermal exposure.Discuss PFAS exposure pathways relevant to ecological receptors; general toxicological information available for invertebrates, vertebrates, and plants; the data gaps and uncertainties associated with ecotoxicological studies; and current approaches in ecological risk assessment of PFAS.Discuss development and findings of some of the External Data Tables including the Water and Soil Values Table, the Air Criteria Table, and the Regulatory Programs Summary Table. Discuss the current status of some of the Federal Regulations including drinking water regulations and how this may impact some states. Discuss the current status of AFFF alternatives and replacement. To view this archive online or download the slides associated with this seminar, please visit http://www.clu-in.org/conf/itrc/PFAS-BTB-HH_042324/