If ligation is more your thing - Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms - Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/ - Quick run-through video by ABI: https://www.youtube.com/watch?v=nlvyF8bFDwM - Ars technica about the technology: https://arstechnica.com/science/2009/12/dna-sequencing-gets-solid-with-built-in-error-detection/ - atdbio explaining SOLiD sequencing: https://atdbio.com/nucleic-acids-book/Next-generation-sequencing#Sequencing-by-ligation-SOLiD - Biogene Blog on SOLiD sequencers: https://www.creative-biogene.com/blog/index.php/2017/02/22/the-next-generation-sequencing-platform-of-abi-company/ Chapters: 01:00 Intro 02:45 Ligation 06:50 Getting hairy balls 20:00 Bead 1 with DNA polonies 23:30 6 Base Jokers 24:00 2-Base encoding colours 29:15 Offsets and holes 35:00 Randomness and barcodes 37:25 Chemistry summary 40:30 Homopolymers are not the problem 46:30 SOLiDs fate Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ). The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).