Multiplexed Föster resonant energy transfer imaging provides a systematic way to study signal networks that govern complex cellular processes. At present, real-time studying of these processes is limited by our inability to imaging the FRET network among four or more fluorescent labels simultaneously. The difficulty in multiplexed FRET rises from the complex photon pathway network in a multilabel FRET complex. To apply multiplexed FRET in live imaging, all photon pathways need to be imaged in parallel. We are developing a novel technique that measures fluorescence lifetime and intensity as images of excitation-emission matrices and fully analyzes all possible photon pathways within a single imaging scan in high speed. Quantitative analysis of lifetime EEM images will allow us to observe FRET between four or more labels in live samples, and therefore reconstruct the molecular machinery behind the multiplexed FRET phenomenon.