Should I Use Constant Current or Voltage in SDS-PAGE?
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#28 — SDS-PAGE gels can be run at constant current, constant voltage, or constant power. But which is best? Listen to this episode of Mentors at Your Benchside to discover the differences between current, voltage, and power, and how they affect how your gels run. Visit the original article for a summary table of running your SDS-PAGE gels at a constant voltage, current, or power. [1] We've also got tips and tricks for casting the perfect SDS-PAGE gel, [2] or read a refresher on how SDS-PAGE works. [3] Resources: 1. Constant Current or Voltage in SDS-PAGE: The Great Debate. Available at: https://bitesizebio.com/51744/constant-current-or-voltage-in-sds-page/. 2. A Simple SDS-PAGE Gel Recipe and 10-Step Casting Protocol for Perfect Gels. Available at: https://bitesizebio.com/59429/sds-page-gel-recipe/. 3. How SDS-PAGE Works. Available at: https://bitesizebio.com/580/how-sds-page-works/.
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