In this episode of Science with a Twist, host Rosy Lee welcomes Joshua J. Coon, a distinguished professor from the University of Wisconsin-Madison and head of Coon Laboratories. They dive into the fascinating world of mass spectrometry and its revolutionary impact on biomedical research. Coon shares his journey from rural Michigan to a leading figure in mass spectrometry, highlighting his passion for both science and instrument building. The conversation shifts to Joshua's groundbreaking work in his lab. He explains how mass spectrometry enables the detailed study of biological systems, from cellular components to complex tissues. Coon's team uses this technology to identify and quantify thousands of molecules, offering deep insights into genetic and lifestyle impacts on molecular behavior in organisms. A significant part of the discussion focuses on how Joshua's research contributes to understanding diseases. By linking specific proteins to metabolic disorders and diseases like Alzheimer's, his work paves the way for potential new treatments and diagnostics. This episode not only sheds light on the technical aspects of mass spectrometry but also underscores its vital role in advancing human health.