Description
l-h" supply of oxygen is absolutely essential
I for the existence of higher organisms. As
the saying goes too much of even the best is
bad. Very high concentrations of 02 are found
to be toxic, and can damage tissues. The present
day concept of oxygen toxicity is due to the
involvement of oxygen free radicals or reactive
oxygen species (ROS). In fact, the generation of
reactive metabolites of 02 is an integral part of
our daily life.
A free radical is defined as a molecule or
a mof ecular species that contains one or
more unpaired electrons, and is capable of
independent existence.
Types of free radicals
Oxygen is required in many metabolic
reactions, particularly for the release of energy.
During these processes, molecular O2 is
completely reduced, and converted to water.
However, if the reduction of 02 is incomplete, a
series of reactive radicals are formed, as shown
in the next column.
r,r,.. (Molecular orygen)
re-
Y
O, (Superoxide)
h€,2H+
J
H rO, (Hydrogen peroxide)
l. e-,H'
Hzo+1
+
OH- (Hydroxyl radicat)
h e-'H*
+
,-r,r'(Water)
Besides the above (O2, H2O2, OH-), the other
free radicals and reactive oxygen species of
biological importance include singlet oxygen
(1O2), hydroperoxy radical (HOO-), lipid
peroxide radical (ROO-), nitric oxide (NO-) and
peroxyn itrite (ONOO-).
The common characteristic features of free
radicals are listed
. Highly reactive
o Very short half-lifeCan generate new radicals by chain reaction
Cause damage to biomolecules, cells and
tissues
Free radicals and reactive oxygen species
(ROS)-not synonymous : By definition, a free
radical contains one or more unpaired electrons.
e.g. Or, OH-, ROO-. There are certain non-
radical derivatives of 02 which do not contain
unpaired electrons e.g. H2O2, 102. The term
reactive oxygen species is used in a broad sense
to collectively represent free radicals, and non-
free radicals (which are extremely reactive) of
the biological systems. However, most authors
do not make a clear distinction between
free radicals and ROS, and use them inter-
changebly.
SOURCES AND GEI{ERATION
OF FREE RADICALS
The major sources responsible for the
generation of free radicals may be considered
under two categories
l. Due to normal biological processes (or
cellular metabolism).
ll. Due to environmental effects.
It is estimated that about 1-4o/o of the 02
taken up by the body is converted to free
radicals. A summary of the sources for
generation of free radicals is given in the
Tahle 34.1 , and a couple of the processes are
briefly described.
Lipid peroxidation
Free radical-induced peroxidation of
membrane lipids occurs in three stages-initiation,
propagation and termination
Initiation phase : This step involves the
removal of hydrogen atom (H) from
polyunsaturated fatty acids (LH), caused by
hydroxyl radical
LH + OH- -----+ L- + H2O
Propagation phase : Under aerobic
conditions, the fatty acid radical (L-) takes up
oxygen to form peroxy radical (LOO-). The
I Cellular metabolism
. Leakage of electrons lrom the respiratory chain
(ETC).
. Productionf HrO, or O, by oxidase enzymes
(e.9. xanthine oxidase, NADPH oxidase).
. Due to chain reactions ofmembrane lipid
peroxidation.
. Peroxisomal generation of O, and HrOr.
. During the synthesis ofprostaglandins.
. Productionf nitric oxide from arginine.
. During the course of phagocytosis (as a pad of
bactedcidal action).
. In the oxidation ofheme to bile pigments.
. As a result of auteoxidation e.g. metal ions
[Fd*, Cu2*]; ascorbic acid, glutathione, flavin
coenzymes.
II Environmental effects
. As a result of drug melabolism e.g. paracetamol,
halothane, cy{ochrome P* rehted reactions.
. Due to damages caused by ionizing radiations
(e.9. X-rays) on tissues.
. Photolysis of O, by light.
. Photoexcitation of organic molecules
. Cigarette smoke contains free radicals, and trace
metals that generate OH-.Alcohol, promoting lipid peroxidation.