All episodes of PaperPlayer biorxiv cell biology

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Identification of omega-3 oxylipins in human milk-derived extracellular vesicles with pro-resolutive actions in gastrointestinal inflammation.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.551608v1?rss=1 Authors: Gomez-Ferrer, M., Amaro-Prellezo, E., Albiach-Delgado, A., Ten-Domenech, I., Kuligowski, J., Sepulveda, P. Abstract: Premature infants (PIs) are at risk of suffering necrotizing enterocolitis (NEC), and infants consuming human milk (HM) show a lower incidence than infants receiving formula. The composition of HM has been studied in depth, but the lipid content of HM-derived small extracellular...

Published 08/05/23

Super-resolution optical imaging reveals accumulation of small mitochondria in the nucleoli of mouse embryos and stem cells

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551824v1?rss=1 Authors: Qin, K., Wu, X. Abstract: The nuclear mitochondrial DNA (NUMT) is found in cancer cells, but the mitochondrial DNAs entering the nuclei in normal cells have not been captured. Here, we utilized super-resolution optical imaging to capture the phenomenon by the probe PicoGreen and found mitochondrial DNAs and mitochondria accumulated in the nucleoli by four probes and overexpressing the...

Published 08/05/23

A fibroblast-derived TGFβ/sFRP2 noncanonical Wnt signaling axis underlies epithelial metaplasia in idiopathic pulmonary fibrosis

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.02.551383v1?rss=1 Authors: Cohen, M. L., Brumwell, A. N., Ho, T. C., Montas, G., Golden, J. A., Jones, K. D., Wolters, P. J., Wei, Y., Chapman, H. A., Le Saux, C. J. Abstract: Reciprocal interactions between alveolar fibroblasts and epithelial cells are crucial for lung homeostasis, injury repair, and fibrogenesis, but underlying mechanisms remain unclear. To investigate this, we administered the fibroblast-selective...

Published 08/05/23

PaperPlayer biorxiv cell biology

Published 08/05/23

Cell growth and nutrient availability control the mitotic exit signaling network in budding yeast

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.552008v1?rss=1 Authors: Talavera, R. A., Prichard, B. E., Sommer, R. A., Leitao, R. M., Sarabia, C. J., Hazir, S., Paulo, J. A., Gygi, S., Kellogg, D. Abstract: Cell growth is required for cell cycle progression. The amount of growth required for cell cycle progression is reduced in poor nutrients, which leads to a reduction in cell size. In budding yeast, nutrients influence cell size by modulating the duration and...

Published 08/04/23

Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complex

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.02.551232v1?rss=1 Authors: Nguyen, T. P., Otani, T., Tsutsumi, M., Fujiwara, S., Nemoto, T., Fujimori, T., Furuse, M. Abstract: Epithelia must be able to resist mechanical force to preserve tissue integrity. While intercellular junctions are known to be important for the mechanical resistance of epithelia, the roles of tight junctions (TJs) remain to be established. We previously demonstrated that epithelial cells devoid...

Published 08/04/23

The mechanics of bronchoconstriction cause airway epithelial damage and inflammation by excess crowding-induced extrusion

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.551943v1?rss=1 Authors: Bagley, D. C., Russell, T., Ortiz-Zapater, E., Fox, K., Redd, P. F., Joseph, M., Rice, C. D., Reilly, C. A., Parsons, M., Rosenblatt, J. Abstract: Asthma is a common disease characterized by airway constriction, excess mucus, and inflammation. Although asthma is an inflammatory disease, subclassed by different endotypes, triggers, and immune responses, the defining diagnostic symptom is...

Published 08/04/23

RBL2 represses the transcriptional activity of Multicilin to inhibit multiciliogenesis

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.551992v1?rss=1 Authors: Quiroz, E. J., Kim, S., Gautam, L. K., Borok, Z., Kintner, C., Ryan, A. L. Abstract: A core pathophysiologic feature underlying many respiratory diseases is multiciliated cell dysfunction, leading to inadequate mucociliary clearance. Due to the prevalence and highly variable etiology of mucociliary dysfunction in respiratory diseases, it is critical to understand the mechanisms controlling...

Published 08/04/23

Optogenetic control of lysosome function

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.02.551716v1?rss=1 Authors: Ilyinsky, N. S., Bukhalovich, S. M., Bagaeva, D. F., Nesterov, S. V., Alekseev, A. A., Tsybrov, F. M., Bogorodskiy, A. O., Moiseeva, O. V., Vlasova, A. D., Kovalev, K. V., Mikhailov, A. E., Rogachev, A. V., Bamberg, E., Ivanovich, V., Borshchevskiy, V. I. Abstract: Lysosome protective, metabolic, signaling functions are highly dependent on their pH. A lack of tools of high spatial and temporal...

Published 08/04/23

Multi-color live-cell optical nanoscopy using phasor analysis

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.551988v1?rss=1 Authors: Zhang, Z., Huang, Y., Tao, W., Wei, Y., Xu, L., Gong, W., Zhang, Y., Han, Y., Kuang, C., Liu, X. Abstract: Stimulated emission depletion microscopy (STED) is a powerful tool for studying nanoscale cell structure and activity, but the difficulties it encounters in multicolor imaging limit its application in biological research. To overcome the disadvantages of limited number of channels and high...

Published 08/04/23

Comprehensive genome editing confers "off-the-shelf" CAR-T cells superior efficacy against solid tumors

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551705v1?rss=1 Authors: Murray, R., Navarrete, N. R. R., Desai, K., Chowdhury, M. R., Chilakapati, S. R., Chong, B., Messana, A., Sobon, H., Rocha, J., Musenge, F., Camblin, A., Ciaramella, G., Sitkovsky, M., Maldini, C., Hatfield, S. Abstract: Biochemical and immunological negative regulators converge to inhibit tumor-reactive Chimeric Antigen Receptor T (CAR-T) cells, which may explain clinical failures of CAR-T cell...

Published 08/04/23

hGRAD - a versatile 'one-fits-all' system for the acute depletion of RNA binding proteins in nuclear condensates

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.551933v1?rss=1 Authors: Muller-McNicoll, M., Zarnack, K., McNicoll, F., Keller, M., Sliskovic, I., Okuda, E. K., Riegger, R. J., Arnold, B. Abstract: Nuclear RNA binding proteins (RBPs) are difficult to study because they often belong to large protein families and form extensive networks of auto- and cross-regulation. They are highly abundant and often localize to condensates with a slow turnover, requiring long...

Published 08/04/23

Positive feedback regulation of Pparγ1sv and Pparγ2 during adipogenesis in 3T3-L1 cells

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551916v1?rss=1 Authors: Takenaka, Y., Kakinuma, Y., Inoue, I. Abstract: We have previously identified the novel splicing variant of mouse Ppar{gamma} (Ppar{gamma}1sv) and proposed the synergistic regulation of the early stage of adipocyte differentiation by Ppar{gamma}1sv and Ppar{gamma}2. Here, we report the finding of PPAR{gamma}-binding sites within the Ppar{gamma} gene locus and its importance in adipogenesis and...

Published 08/04/23

A force-sensitive mutation reveals a spindle assembly checkpoint-independent role for dynein in anaphase progression

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551815v1?rss=1 Authors: Salvador-Garcia, D., Jin, L., Hensley, A., Golcuk, M., Gallaud, E., Chaaban, S., Port, F., Vagnoni, A., Planelles-Herrero, V. J., McClintock, M. A., Derivery, E., Carter, A. P., Giet, R., Gur, M., Yildiz, A., Bullock, S. L. Abstract: The cytoplasmic dynein-1 (dynein) motor organizes cells by shaping microtubule networks and moving a large variety of cargoes along them. However, dynein's diverse...

Published 08/04/23

FAM210A mediates an inter-organelle crosstalk essential for protein synthesis and muscle growth in mouse

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551853v1?rss=1 Authors: Chen, J., Yue, F., Kim, K. H., Zhu, P., Qiu, J., Tao, W. A., Kuang, S. Abstract: Mitochondria are not only essential for energy production in eukaryocytes but also a key regulator of intracellular signaling. Here, we report an unappreciated role of mitochondria in regulating cytosolic protein translation in skeletal muscle cells (myofibers). We show that the expression of mitochondrial protein...

Published 08/04/23

Aggregation of Disrupted in Schizophrenia 1 arises from a central region of the protein

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551216v1?rss=1 Authors: Zaharija, B., Bradshaw, N. J. Abstract: An emerging approach to studying major mental illness is through proteostasis, with the identification of several proteins that form insoluble aggregates in the brains of patients. One of these is Disrupted in Schizophrenia 1 (DISC1), a neurodevelopmentally-important scaffold protein, and the product of a classic schizophrenia risk gene. DISC1 was seen to...

Published 08/04/23

Recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.550647v1?rss=1 Authors: Ibtisam, I., Kisselev, A. F. Abstract: Rapid recovery of proteasome activity may contribute to intrinsic and acquired resistance to FDA-approved proteasome inhibitors. Previous studies have demonstrated that the expression of proteasome genes in cells treated with sub-lethal concentrations of proteasome inhibitors is upregulated by the transcription factor Nrf1 (NFE2L1), which is activated by a...

Published 08/04/23

A Bright, Photostable Dye that Enables Multicolor, Time Lapse, and Super-Resolution Imaging of Acidic Organelles

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.552058v1?rss=1 Authors: Lesiak, L., Dadina, N., Zheng, S., Schelvis, M., Schepartz, A. Abstract: Lysosomes have long been known for their acidic lumen and efficient degradation of cellular byproducts. In recent years it has become clear that their function is far more sophisticated, involving multiple cell signaling pathways and interactions with other organelles. Unfortunately, their acidic interior, fast dynamics,...

Published 08/04/23

Oligomerization and feedback on membrane recruitment stabilize PAR-3 asymmetries in C. elegans zygotes.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.552031v1?rss=1 Authors: Lang, C. F., Anneken, A., Munro, E. Abstract: The PAR polarity network is a paradigmatic example of how systems of mutually antagonism interactions among peripheral membrane binding proteins allow them to form and maintain complementary polar domains in response to a transient polarizing cue. The oligomeric scaffolding protein PAR-3 has emerged as a keystone member of the PAR network in many...

Published 08/04/23

Determining the structure of protein-bound ceramides, essential lipids for skin barrier function

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551778v1?rss=1 Authors: Ohno, Y., Nakamura, T., Iwasaki, T., Katsuyama, A., Ichikawa, S., Kihara, A. Abstract: Protein-bound ceramides, specialized ceramides covalently bound to corneocyte surface proteins, are essential for skin permeability barrier function. However, their exact structure and target amino acid residues are unknown. Here, we found that epoxy-enone (EE) ceramides, precursors of protein-bound ceramides,...

Published 08/04/23

Correlative single-cell X-ray tomography and X-ray fluorescence imaging

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551868v1?rss=1 Authors: Lin, Z., Zhang, X., Nandi, P., Lin, Y., Wang, L., Chu, Y., Paape, T., Yang, Y., Xiao, X., Liu, Q. Abstract: X-ray tomography and x-ray fluorescence imaging are two non-invasive imaging techniques to study cellular structures and chemical element distributions, respectively. However, correlative X-ray tomography and fluorescence imaging for the same cell has yet to be routinely realized due to...

Published 08/04/23

Discovery of a multipotent cell type from the term human placenta

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.02.551028v1?rss=1 Authors: Vadakke-Madathil, S., Wang, B., Oniskey, M., Dekio, F., Brody, R., Gelber, S., Sperling, R., Chaudhry, H. W. Abstract: The human placenta is a reservoir of a multitude of cell types with immense regenerative potential. Caudal-type homeobox-2 (CDX2) is a conserved factor that regulates trophectoderm formation and placentation during early embryonic development and hence can play a vital role in...

Published 08/04/23

Parallel proteomics and phosphoproteomics defines starvation signal specific processes in cell quiescence

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551843v1?rss=1 Authors: Sun, S., Tranchina, D., Gresham, D. Abstract: Cells arrest growth and enter a quiescent state upon nutrient deprivation. However, the molecular processes by which cells respond to different starvation signals to regulate exit from the cell division cycle and initiation of quiescence remains poorly understood. To study the role of protein expression and signaling in quiescence we combined...

Published 08/04/23

Comprehensive genome editing confers "off-the-shelf" CAR-T cells superior efficacy against solid tumors

Published 08/04/23

Presence of the ER-Golgi intermediate compartment that matures into the Golgi in yeast

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551802v1?rss=1 Authors: Tojima, T., Suda, Y., Jin, N., Kurokawa, K., Nakano, A. Abstract: Cargo traffic through the Golgi apparatus is mediated by cisternal maturation, but it remains largely unclear how the cis-cisternae, the earliest Golgi sub-compartment, is generated. Here, we use high-speed, high-resolution confocal microscopy to analyze the spatiotemporal dynamics of a diverse set of proteins that reside in and...

Published 08/03/23